瑞典实验室研究发现辉瑞疫苗中的mRNA会整合进入人体细胞DNA [复制链接]

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发表于 2022-3-4 21:13:07 |只看该作者 |倒序浏览 微信分享

一瑞典实验室研究发现辉瑞疫苗中的mRNA会整合进入人体细胞DNA，并改变受影响细胞的DNA。这一研究结果发表在Current Issues in Molecular Biology杂志上.
他们采用了人类肝细胞系进行体外实验，发现辉瑞mRNA疫苗使用LINE-1天然逆转录酶，将疫苗的遗传密码逆转录整合人体细胞DNA。各位有兴趣研究的野生医学爱好者，可查阅。

论文链接：https://www.riotimesonline.com/wp-content/uploads/2022/02/Pfizer-RNA-into-DNA.pdf

Abstract: Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer andBioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection.Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. In this study, we investigated the effect of BNT162b2 onthe human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitativePCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is anendogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 openreading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicatedincreased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We alsoshow that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h uponBNT162b2 exposure.

注射疫苗后，六小时既可在肺细胞发现DNA改变。

5. ConclusionsOur study is the first in vitro study on the effect of COVID-19 mRNA vaccine BNT162b2on human liver cell line. We present evidence on fast entry of BNT162b2 into the cells andsubsequent intracellular reverse transcription of BNT162b2 mRNA into DNA.

结论是：通关观察逆转录酶，BNT 疫苗可改变人体肺细胞DNA.

Funding: This study was supported by the Swedish Research Council, Strategic Research Area Exodiab, Dnr 2009-1039, the Swedish Government Fund for Clinical Research (ALF) and the foundationof Skåne University Hospital

该试验资助由瑞典政府医学研究会和 Skåne University Hospital基金会提供。

以上仅是节选翻译，一切内容以原文为准。

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